Title: Cholecystokinin octapeptide modulates Th1/Th2 imbalance in splenocytes of KLH-immunized mice
Abstract: AIM:To investigate the effects of cholecystokinin octapeptide (CCK-8) on Th1/Th2 imbalance. METHODS: Female BALB/c mice were treated with CCK-8 and keyhole limpet haemocyanin (KLH), splenocytes were acquired and incubated in vitro with KLH restimulation, the productions of Th1 cytokines, interferon (IFN)-γ, interleukin (IL)-2 and Th2 cytokines, IL-4, IL-5 in cell culture supernatants were detected by enzyme-linked immunosorbnent assay (ELISA). The mRNA expressions of IFN-γ, IL-2, IL-4 and IL-5 in splenocytes were detected by reverse transcription-polymerase chain reaction (RT-PCT). The levels of Th1 antibody IgG2a and Th2 antibodies IgG1 in serum were also detected by ELISA. RESULTS: ① Splenocytes derived from KLH immunized mice produced much higher level of IL-5, IL-4, IL-2 and IFN-γ and expressed much more mRNA than those in control mice. The IL-4/IFN-γ ratio was elevated, in vivo treatment of CCK-8 made KLH immunized splenocytes produced much higher IFN-γ and IL-2, and expressed much more IFN-γ and IL-2 mRNA, but made IL-4 and IL-5 productions and mRNA expressions reduced. ② The levels of KLH specific antibody IgG2a and IgG1, especially IgG1 were elevated in serum of KLH-immunized mice. In vivo administration of CCK-8 reduced IgG1 production and heightened IgG2a production. CONCLUSION: CCK-8 enhances Th1 response and reduces Th2 response in KLH-immunized mice in vivo, suggesting that CCK-8 regulates adaptive immune response and it may be a useful regulator in Th1/Th2 imbalance.
Publication Year: 2009
Publication Date: 2009-01-01
Language: en
Type: article
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Cited By Count: 2
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