Title: Cloning and Sequencing of Major Allergenic Gene cDNA from Tartary Buckwheat
Abstract: The cDNA sequence of allergenic gene expresses a kind of 22 kD protein in tartary buckwheat (TB), was obtained using RT PCR and 3′ rapid amplification of cDNA ends (3′RACE). The cDNA sequence coding for this allergenic protein, consisted of 768 nucleotides, including 588 nucleotides in the open reading frame (ORF) and 180 nucleotides in the 3′ untranslated region. The ORF encoded a functional protein of 195 amino acids. Comparing this allergy from TB with those of common buckwheat (CB), it showed that this allergy in TB shared 95% and 93% nucleotide sequence homology with allergenic protein and legumin like protein in CB, respectively. The protein sequence of TB allergenic protein shared 93% and 83% homology with those of globulin protein in CB and sword bean protein, respectively. The conserved sequence KEEEKE at position 183 188 was probably considered to be the antigen determinant sequence similar to that of other allergenic proteins found in food and pollen.
Publication Year: 2003
Publication Date: 2003-01-01
Language: en
Type: article
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