Title: Mechanism of Rhizobacteria BH_1 (Bacillus sp.) to Suppress Soybean Root Rot Disease Caused by Fusarium spp.
Abstract: Strain BH1 (Bacillus sp.), isolated from soybean rhizosphere, is of potential in biocontrol of soybean root rot disease (SRRD) caused by Fusarium spp. based on greenhouse tests. In field trials, control efficacy of SRRD by BH1 was up to 561%. The mechanism of BH1 to suppress SRRD was conducted. The spores germination and hyphae growth of pathogens F3 (Fusarium oxysporum), W1 (F. solani) and S1 (F. sp.) were inhibited by BH1 fermentation filtrate. The affinity of BH1 with soybean roots was examined. BR+4, a mutant resistant to rifampicin, could colonize soybean rhizosphere when soybean were planted in sterilized soil or soil infested with F. oxysporum. The dynamics of BR+4 was similar in both infested and sterilized soils, but the amount of BR+4 was higher in sterilized soil than that in infested soil. The pressprinting examination showed that BR+4 had a high affinity with soybean roots, and could grow along with the roots. SEM observations indicated that BR+4 aggregated in the indented parts of the epidermal surface, few on root tip or lateral roots, and no in intracellular gap. The results indicate that competition for nutrition and space is an important mechanism for BH1 to suppress soybean root rot diseases caused by Fusarium spp. in soil.
Publication Year: 2003
Publication Date: 2003-01-01
Language: en
Type: article
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Cited By Count: 1
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