Title: Effect of recombinant human glucagon-like peptide-1 on plasma glucose in the experimental diabetic rats
Abstract: PurposeTo study the effects of two recombinant human glucagon-like peptide-1 [rhGLP-1(7- 36)NH 2 and rhGLP-1(7-37)] on plasma glucose and insulin in the experimental diabetic SD rats.MethodsSD rat model with hyperglycemia was developed by intraperitoneal injection in these normal SD rats with 50%glucose 3.24 g/kg. rhGLP-1(7-36)NH 2 and rhGLP-1(7-37) were administered at different doses, then blood samples were collected and the levels of plasma glucose and insulin were determined. Subsequently, the experimental 1 model diabetic rats were produced with streptozotocin on 60 mg/kg. rhGLP-1(7-36)NH 2 [4.0 ng/(kg·min)] was intravenously injected into the diabetic rats for 120 min and the levels plasma glucose was measured.ResultsCompared with glucose group, plasma glucose levels were significantly decreased after administration 40,80 μg/kg rhGLP-1(7-36)NH 2 and rhGLP-1(7-37) in the experimental SD rat model. Besides, the levels of insulin in plasma were also apparently increased in these rats as compared with glucose rats (P0.001). Both rhGLP-1(7-36)NH 2 and rhGLP-1(7-37) could improve the levels of plasma glucose and insulin, but these indexes showed no significant difference between them. In the type 1 diabetic rats, plasma glucose concentrations were(17.04±1.31) mmol/L (P0.05) after 60 min by intravenous injection rhGLP-1(7-36)NH 2, and glucose concentrations were(11.98±1.05) mmol/L (P0.001) after 120 min.ConclusionrhGLP-1 treatment could improve plasma glucose levels in the experimental diabetic rats model. It might be related to stimulating the secretion of plasma insulin in the rats model.
Publication Year: 2004
Publication Date: 2004-01-01
Language: en
Type: article
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