Title: Suppression of RNA Interference on Expression of Skp2 of SKOV3 Ovarian Carcinoma Cell Line
Abstract: Objective: To investigate suppression of RNA interference (RNAi) on expression of Skp2 of SKOV3 ovarian carcinoma cell line. Methods: The Skp2-siRNA was designed and synthesized, then transfected to SKOV3 ovarian carcinoma cell lines. The cell lines were divided into five groups, including the blank control group, the siRNA transfection group 1, the siRNA transfection group 2, the mock transfection group and the negative control group. The expression level of Skp2 mRNA and protein were detected by realtime-PCR and Western blotting, respectively. The growth and proliferation of SKOV3 ovarian carcinoma cell lines were observed with CCK-8 assay. Results: After transfection with Skp2-siRNA, the expression level of Skp2 mRNA and protein were down-regulated, the growth and proliferation of SKOV3 ovarian carcinoma cell line were inhibited in the siRNA transfection group 1 and the siRNA transfection group 2. There were significant differences between the siRNA transfection group 1 and group 2 and the blank control group(P 0.05),but no significant difference between the siRNA transfection group 1 and siRNA transfection group 2 (P 0.05). The silencing efficiencies were 75.31% and 76.86% in siRNA transfection group 1 and group 2 respectively. The protein suppression rates were 62.10% and 63.11% respectively. The inhibition ratios were 52.75% and 53.06% respectively by CCK-8 assay. Conclusion: The down-regulation of Skp2 expression of SKOV3 ovarian carcinoma cell line by Skp2-siRNA can lead to the suppression of proliferation. The small interfering RNAs technique can inhibit the proliferation of carcinoma cell by oncogene silencing.
Publication Year: 2010
Publication Date: 2010-01-01
Language: en
Type: article
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