Title: Effects of dendritic cells stimulated with C5b-9 on allogenic lymphocytes
Abstract: Objective To study the effect of dendritic cells(DC) stimulated with C5b-9 on allogenic lymphocytes. Methods DC was stimulated with C5b-9.CD4+ T and CD8+ T cells were isolated by magnetic-activated cell sorting (MACS),and were cocultivated with DC.The maturation markers and cytokine secretion of T cells were analyzed by flow cytometry and ELISA.Results The high pure CD4+ T and CD8+ T cells were separated successfully.After cocultivated with DC,the muturation markers CD25 and CD69 of CD4+ T were up-regulated by 26.31% and 52.73%,and IL-2 and IFN-γ of CD4+ T cells were promoted from 126.3 ng·L-1 and 156.7 ng·L-1 to 409.2 ng·L-1 and 471.5 ng·L-1;the levels of IL-10 before and after coculture were 104.3 ng·L-1 and 107.1 ng·L-1.However these markers and cytokine secretion of CD8+ T were not influenced.Conclusion C5b-9 can adjust the function of DC and regulate the immuological response of T cells.
Publication Year: 2008
Publication Date: 2008-01-01
Language: en
Type: article
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