Title: THE RELATIONSHIP OF INCREASED INTRACELLULAR S-ADENOSYLHOMOCYSTEINE WITH HUMAN UMBILICAL VEIN ENDOTHELIAL CELLS DAMAGE AND THE WHOLE DNA METHYLATION
Abstract:Objective:To explore the relationship of the damage induced by intracellular increased s-adenosylhomocysteine(SAH) and the whole DNA methylation in human umbilical vein endothelial cells(HUVEC) . Meth...Objective:To explore the relationship of the damage induced by intracellular increased s-adenosylhomocysteine(SAH) and the whole DNA methylation in human umbilical vein endothelial cells(HUVEC) . Method:After HUVEC were treated with different concentrations of potent s-adenosylhomo-cysteine hydrolase(SAHH) inhibitor 3-deazaadenosine(DZA) for 24,48 and 72h,the cell morphology and cellular ultrastructure were observed with light microscope and eletron microscope respectively. The cellular SAH level was measured with reversed phase high-performance liquid chromatography(RP-HPLC) . The homocysteine(Hcy) concentration in medium was determined by fluorescence polarization immunoassay(FPIA) . The cell growth and proliferation were determined by MTT assay. The apoptotic percentage was analyzed with flow cytometry. The whole genome methylation was analyzed with cytosine extensionmethod. Results:Compared to normal,the HUVEC after treated with DZA emerged the apoptosis and necrosis characters. DZA up-regulated the intracellular SAH level,down-regulated Hcy concentration in medium,and inhibited the celluar growth and proliferation. The level of whole genome methylation in DZA treated group was also lower than that of normal group. Conclusion:The intracellular increased SAH damages the HUVEC,which is correlated with whole DNA methylation. It's possible that SAH is the potential biological marker in atherogenesis.Read More
Publication Year: 2007
Publication Date: 2007-01-01
Language: en
Type: article
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