Title: Optimization of Culture Medium for Heavy-ion Irradiation Bread Yeast Design
Abstract: A mutant bread yeast strain with high protein content of 55% was gained by use of 12C6+ ions.The MINITAB 16.0 software,Plackett-Burman experimental design and response surface methodology were applied to optimize the culture medium for the irradiated yeast.The most important three factors which influenced the culture results were identified as glucose,magnesium sulphate and yeast extract.The path of the steepest ascent was undertaken to approach the optimal region of the three significant factors.Box-Behnken design and response surface methodology were used for the regression analysis.Finally,the optimal fermentation conditions were identified as glucose 11.03 g/L,yeast extract 6.53 g/L and magnesium sulphate 5.59 g/L by the regression analysis.It was found that the biomass of the bread yeasts reached 4.84 g/L and increased by 15% compared to original conditions.
Publication Year: 2013
Publication Date: 2013-01-01
Language: en
Type: article
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