Title: Effects of propofol or midazolam combined with fentanyl on GABA_A receptor in hippocampal pyramidal neurons of rats
Abstract: Objective To observe the effects of propofol or midazolam combined with fentanyl on GABA_A receptor in acutely dissociated hippocampal pyramidal neurons of neonatal rats, so as to reveal the possible mechanism of the combined anesthesia. Methods Whole-cell patch-clamp technique was used to record the GABA_A receptor-induced currents (I_GABA) in four groups(n=5 of each), ie, propofol(groupⅠ), propofol combined with fentanyl(groupⅡ), midazolam (groupⅢ) and midazolam combined with fentanyl(groupⅣ). Results 0.3-30.0 μmol/L propofol (groupⅠ,Ⅱ) and 0.03-100.0 μmol/L midazolam (groupⅢ, Ⅳ) facilitated I_GABA with the greatest enhancement at 3.0 μmol/L of each (P0.05). Following 0.01 μmol/L fentanyl pretreatment for 10 minutes, the facilitation of I_GABA by 3.0 μmol/L propofol increased significantly [(127.2±11.2)% (groupⅠ) vs (212.5±14.9)% (groupⅡ), P0.05, n=5], whereas the effects of midazolam were not changed by fentanyl pretreatment [(158.2±34.0)% (groupⅢ) vs (116.3±46.0)% (groupⅣ), P0.05, n=5]. Conclusion Fentanyl can enhance the effects of propofol on GABA_A receptor, but not influence those of midazolam. Propofol or midazolam combined with fentanyl may prevent the neuroexcitatory effects of fentanyl, supporting their use in combination for clinical anesthesia.
Publication Year: 2005
Publication Date: 2005-01-01
Language: en
Type: article
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