Title: Expression of EGFP Gene in Goat Fetal Fibroblast Cells
Abstract: In order to establish the goat fetal fibroblasts cell(gFFC)clones stably expressing the enhanced green fluorescent protein(EGFP)gene,the goat fetal fibroblast cells(gFFC)were harvested by tissue explants culture method and then transfected with the EGFP gene using the QIAGEN transfection reagent.The positive cells with transgenes were screened using different concentrations of G418.The results showed that the propagation of gFFC was normal and the highest transfection efficiency was achieved when using the 70%-80%confluent cells in the 24-well plate cultured with supplement of 40μL transfection medium,which contains 0.27μg DNA and 2μl QIAGEN transfection reagent.One month later,the drug-resistant cell clones gradually formed under the selection of G418.The results indicated that the EGFP was expressed efficiently in gFFC.
Publication Year: 2013
Publication Date: 2013-01-01
Language: en
Type: article
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