Title: Establishment and application of the multiplex PCR to the detection of CSF,PP,PRRS and AD viruses
Abstract: classical swine fever virus(CSFV),porcine parvoviruses(PPV),porcine reproductive and respiratory syndrome virus(PRRSV) and Aujeszky's disease virus(ADV) all can result in reproductive failure of swine,which causes significant economical losses to the swine industry on a world-wide scale.Four primers were designed and the multiplex PCR were developed.Its sensitivity were CSFV 10 TCID50,PPV 10 TCID50,PRRSV 1 TCID50,PRV 100 TCID50 CSFV 10 TCID50,PPV 10 TCID50,PRRSV 1 TCID50,PRV 100 TCID50.The multiplex PCR for CSFV Shimen strain,CSFV lapinized virus strain,PRV Minnan A strain,PPV,PRRSV ky35 strain and B13 strain were done by this method.The DNA fragment were obtained from the six viral strains but not from BVDV and BDV.These results showed that the development of the multiplex PCR is very important for early and fast diagnosis of these diseases in china.
Publication Year: 2007
Publication Date: 2007-01-01
Language: en
Type: article
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