Title: Experimental study of intraoperative fluorescence imaging for brain glioma
Abstract:Objective To investigate the feasibility of real time fluorescence imaging during glioma surgery by fluorescence-labeled albumin.Methods Rat serum albumin(RSA) was labeled with sodium fluorescein(NaF)...Objective To investigate the feasibility of real time fluorescence imaging during glioma surgery by fluorescence-labeled albumin.Methods Rat serum albumin(RSA) was labeled with sodium fluorescein(NaF) by chemical synthesis method.In vitro experiments:C6 glioma cells and astrocytes were incubated with 0.1 mg/ml fluorescent protein for 24 h respectively,and then the expression of fluorescence on the cells was observed.In vivo experiments:the intravenous injection of NaF-RSA solution was given 24 h before rat glioma surgery.The appearances of glioma and normal brain tissues were observed during the surgery under sunlight and fluorescence microscope,and the biopsy of the fluorescent tissues was performed and examined by HE staining.Results C6 glioma cells had bright green fluorescence,while astrocyte cells had slender fluorescence in vitro experiment.The in vivo experiment showed that the glioma tissues were stained yellow under sunlight and green fluorescence under fluorescence microscope,while the normal brain had no fluorescence.HE staining showed that the tissues with green fluorescence were glioma tissues.Conclusion The real time fluorescence imaging technology established by fluorescent albumin could effectively distinguish tumor tissues from normal brain tissues during brain glioma surgery.Read More
Publication Year: 2009
Publication Date: 2009-01-01
Language: en
Type: article
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