Title: RAPD Analysis of Thirty-eight Cymbidium ensifolium Cultivars
Abstract: DNA from thirty-eight Cymbidium ensifolium cultivars was examined using polymerase chain reaction(PCR) to determine the efficiency of randomly amplified polymorphic DNA(RAPD) markers in genetic diversity and genetic relationship.A total of 116 RAPD markers,88.79% of which were polymorphic,were produced from 18 arbitrary primers of 10 bp.Genetic distances among the cultivars were estimated based on the amount of band sharing and ranged from 0.0420-0.5385 with an average of 0.2902.Unweighted pair-group method with arithmetic averaging(UPGMA) of genetic distances estimates grouped 'suxin' cultivars and 'caixin' cultivars together with each other,and 'suxin' cultivars diverged from 'caixin' cultivars,thereby agreeing with known traditional classification information.Four cultivars,'Minxi Yuchen','Yuchen Dagong','Yuchen' and 'Yinbian Yuchen' could be distinguished from all the rest based only on the S153-650 bp fragment,and 'Shiliu Luohan' and 'Yuchen' based only on the S38-1 200 bp fragment.'Ma'er Siji' could be differentiated from all the rest by lack of the S38-800 bp fragment.
Publication Year: 2008
Publication Date: 2008-02-25
Language: en
Type: article
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