Title: Development of the subunit vaccine against Mycoplasmal Pneumonia of swine
Abstract: To construct a multi-subunit vaccine that could provide favorable immunologic efficacy,which provides basic data for the research of developing novel vaccines of Mycoplasmal Pneumonia of swine.The main immune original proteins of Mycoplasma hyopneumoniae p36,p46,p65 and p97R1-Nrdf were recombinantly expressed by using molecular cloning,directed mutations and restructuring expression techniques.Using the protein of p36,p46 and p65 as group Ⅰ,p36,p46,p65 and p97R1-Nrdf as group Ⅱ,using the Mycoplasma hyopneumoniae bacterin(M+PAC) as group Ⅲ,PBS added with adjuvants as the control group to immunize BALB/c mice.Detect serum,lungs and the splenic lymphocytes which is stimulated by protein with Mycoplasma pneumoniae antibody,IFN-gamma and IL-4.The results suggested that group Ⅱ induced significantly higher antibody than group Ⅰ and group Ⅲ(P0.01) and also induced significantly higher IFN-γ production than group Ⅲ(P0.05),however,there were no significant differences between group Ⅱ and group Ⅰ or group Ⅰ and group Ⅲ(P0.05).IL-4 production was similar among the three group(P0.05),but was obviously higher than the control group(P0.01).The antibody level of MP antibody,IFN-γ and IL-4,in lung was group Ⅱ,group Ⅰ,group Ⅲ and the control group in proper order.The splenic lymphocytes results showed that the highest1 MP antibody and IFN-γ level were induced by group Ⅱ,while the highest IL-4 production was induced by group Ⅲ.It shows that the recombinant subunit vaccine containing p36,p46,p65 and p97R1-Nrdf provided the highest antibody level by simultaneously stimulating humoral immunity and cell immunity.Group Ⅰ provided almost the same significantly immunologic efficacy as group Ⅲ in the same way.
Publication Year: 2012
Publication Date: 2012-01-01
Language: en
Type: article
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