Title: Laboratory surveillance on poliovirus from 2000 to 2009 in Guizhou Province
Abstract: OBJECTIVE To summary and analyses the work experience of the polio virological surveillance in Guizhou Province and provide the virological surveillance basis for maintaining polio-free status.METHODS The methods of virus isolation,virus identification and nucleotide sequencing were adopted for the virological examination and analysis was made on the result of polio virus surveillance of AFP cases,AFP contacts and healthy crowd by collecting stool specimens in 2000-2009.RESULTS ① In 2000-2009,a total of 3,012 AFP cases were reported in Guizhou Province,and a total of 6,609 part stool specimens were collected from 3,207 reported AFP cases,AFP contacts and healthy crowd.② 275 polioviruses(PV)strains had been isolated form all stool specimens with a virus isolation rate of 6.7%,and 484 non-polio enteroviruses(NPEV)strains had been isolated form all stool specimens with a virus isolation rate of 11.8%.③ 275 strains PV were identified by the National Poliomyelitis Laboratory.Among them,225 strains were vaccine-associated poliovirus,44 strains were vaccine-varied poliovirus,and 6 strains were vaccine-derived poliovirus(VDPVs)(5 strains were type ⅠVDPVs,1 strains were type Ⅱ VDPVs,and all of them were isolated in 2004).It has been 16 years that we haven,t found wild-type poliovirus by the end of 2009 year.④ In 2000-2009,our province polio laboratory successfully passed the yearly Proficiency Test and every 2-3 years Field Accredited of WHO and National Poliomyelitis Laboratory.CONCLUSION Surveillance system of province polio laboratory in Guizhou is running well,and plays well the role of polio virology surveillance.Surveillance system timely discovery and dispose of the vaccine-derived poliovirus strain(VDPVs)circulation,and do not find wild-type poliovirus.Guizhou province achieves the goal of maintaining Polio-free status.
Publication Year: 2012
Publication Date: 2012-01-01
Language: en
Type: article
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