Title: THE SCREENING OF LIPASE-PRODUCING STRAIN AND THE PREPARATION OF ITS PROTOPLAST
Abstract: To obtain lipase\|producing strains under room temperature, we adopted the differential medium and agar block selective method with indicators of Victoria blue and Rhodamine\|B to isolate strains from soil samples. A lipase\|producing fungal strain was screened from oily soil and was dentified as Geotrchum Sp(GS). In order to increase the production of lipase, we made physical and chemical mutagenetic treatments with protoplasts. We found that many protoplasts could be obtained through the treatment with the solution of DTT, mixed enzymes and especially 0.6 mol/L MgSO\-4.
Publication Year: 2003
Publication Date: 2003-01-01
Language: en
Type: article
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