Title: Evolution Analysis of c3h cDNA Fragment from Poplar
Abstract: Coumarate-3-hydroxylase is one of important lignin synthetases.Based on cDNA sequence conserved domain of CYP98A3 (accession number AY064170),a pair of primers were designed and used to amplify a fragment of c3h gene by RT-PCR from poplar(Populus×euramericana cv.‘74/76’)developing second xylem RNA,then cloned into pMD20-T vector,identified by restriction enzyme cutting,PCR amplification and sequencing.The sequence of the amplified DNA fragment was 994 bp in size and contained a 495 bp ORF(open reading frame).Alignment with amino acid sequence of c3h retrieved from NCBI (accession number:AY064170)showed that the similarity was 91%.Therefore,the cloned DNA fragment could primarily classified as a member of the CYP98A3 family.It was submitted to GenBank,the accession number was AM920690.Phylogenetic tree was constructed based on c3h genes from GenBank,and the evolution was also analyzed.
Publication Year: 2008
Publication Date: 2008-01-01
Language: en
Type: article
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