Title: Construction of eukaryotic expression vector carrying rat GLUT1 gene and its expression in 293 cells
Abstract: AIM: To construct a eukaryotic expression vector carrying rat GLUT1 gene. METHODS: GLUT1 cDNA was obtained by RT - PCR technique from rat cerebral tissue and was inserted into eukaryotic expression vector pcDNA3. 1 ( + ) to construct the recombinant expression plasmid pcDNA3.1(+)-GLUT1. The recombinant plasmid was transfected into 293 cells by lipofectamine TM 2000. The transcription and the expression of GLUT1 in 293 cells were tested by RT-PCR and immunocyto-chemistry, respectively. RESULTS: The transcription of GLUT1 gene and expression of GLUT1 protein in transfected 293 cells were observed by RT-PCR and immunocytochemistry. CONCLUSION: The eukaryotic expression vector pcDNA3.1 (+)-GLUT1 is constructed successfully and it can express GLUT1 protein in 293 cells.
Publication Year: 2005
Publication Date: 2005-01-01
Language: en
Type: article
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