Title: Induction and in vitro Culture of Echinacea angustifolia Hairy Roots
Abstract:Objective To induce hairy roots of Echinacea angustifolia by two strains of Agrobacterium rhizogenes A4 and R1000 and establish an in vitro culture system of the hairy roots.Methods Hairy roots were i...Objective To induce hairy roots of Echinacea angustifolia by two strains of Agrobacterium rhizogenes A4 and R1000 and establish an in vitro culture system of the hairy roots.Methods Hairy roots were induced by coculture Effects of explants,Agrobacterium rhizogenes,preculture time along with infecting time on the induction rate and effects of different basic media on growth of hairy root were studied.Results The highest induction rate was obtained from leaves with 48h preculture which were induced by R1000 for 10min.The growth of hairy root could be raised by 1 /2MS medium with 0.5 mg / L IBA.Conclusion Establishment of Echchina angustifolia hairy root culture can provide a foundation for the industrial production of active drug component.Read More
Publication Year: 2013
Publication Date: 2013-01-01
Language: en
Type: article
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