Title: Purification of Melilotus officinalis by macroporous resins
Abstract: Objective To investigate the optimum purification process of the active ingredients rutin, clovin, and coumarin in Melilotus officinalis by macroporous resins. Methods The various types of resins were selected with purification of the active ingredients rutin, clovin, and coumarin in M. officinalis as index. The parameters, such as sample loading maximum volume, sample loading velocity, elution solvent concentration, and elution solvent volume of optimized resin were studied. Results HPD-300 type macroporous resin was selected as the best type of resin for the purification of active ingredients in M. officinalis. The optimum purification conditions were as following: the sample loading maximum volume was 2.4 g/g macroporous resin, sample loading velocity was 2.0 BV/h, and the sample was eluted by water with 2 BV and 50% ethanol with 7 BV, respectively. Rutin, clovin and coumarin proportion promoted 7.8 times after the purification HPD-300 by macroporous resin, and the yield was 85.38%. Conclusion HPD-300 macroporous resin is suitable for the purification of rutin, clovin, and coumarin in M. officinalis with high stability and good repeatability.
Publication Year: 2015
Publication Date: 2015-01-01
Language: en
Type: article
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