Title: Changes of Caspase-3 and Caspase-9 Activity during the Simvastatin-induced Apoptosis in K562 Cells
Abstract: Objective To observe the variations of Caspase-3 and Caspase-9 activities in the process of simvastatin-induced apoptosis in K562 cells.Methods K562 cells were exposed to different concentrations of simvastatin,cell morphological analysis and flow cytometry were performed to confirm cell apoptosis.Colorimetric method was used to measure Caspase-3 and Caspase-9 activities.Results K562 cells could be induced to undergo apoptosis after 5,10,20μmol/L simvastatin treatment for 48h,and the apoptotic rate was( 4.00 ± 0.13 )%,( 6.24 ± 0.18 )% and ( 9.41 ± 0.22 )% respectively,compared with the control group,it was significantly higher(P 0.01 ).At 72h,K562 cells were apoptotic with a rate of ( 7.62 ± 0.21 )%,( 12.41 ± 0.32 )% and ( 19.08 ± 0.26 )% respectively,compared with the control group,it was markedly higher(P 0.01 ).Furthermore,Caspase-3 and Caspase-9 activity in K562 cells elevated remarkably after 10,20μmol/L simvastatin treatment for 48h and 72h,compared with the control group,it was markedly higher(P 0.01 ).Conclusion K562 cells can be induced to undergo apoptosis by simvastatin,the underlying mechanism might be related to upregulation of Caspase-9 activity which subsequently transforms Caspase-3 into its active form.
Publication Year: 2007
Publication Date: 2007-01-01
Language: en
Type: article
Access and Citation
AI Researcher Chatbot
Get quick answers to your questions about the article from our AI researcher chatbot