Title: Effects of Vascular Activators on Expression of VEGF Gene in Human Umbilical Vein Endothelial Cells under Hypoxia
Abstract:To investigate the regulation and expression of VEGF gene in human umbilical vein endothelial cells under hypoxia and vascular activators,such as endothelin(ET),nitric oxide (NO) and NO synthesis inhi...To investigate the regulation and expression of VEGF gene in human umbilical vein endothelial cells under hypoxia and vascular activators,such as endothelin(ET),nitric oxide (NO) and NO synthesis inhibitor N\|nitro\| L \|arginine(L\|NNA),the human umbilical vein endothelial cells were cultured and then treated by hypoxia and vascular activators.The expression on VEGF mRNA level and protein level were assayed by Northern blots,ELISA and computer figure analysis system respectively.When human umbilical vein endothelial cells were subjected to hypoxia for 6 hours the expression of VEGF begans to increase.when vascular activators were added to the 6\|hour group,ET could increase inducement and NO inhibited it and L\|NNA stimulated the cells to express the VEGF.VEGF protein concentration in the cell culture media matched the mRNA result.They were 6 hours hypoxia 8.2±1.1 ng/L,ET+6 hours hypoxia 9.37±1.02 ng/L,NO+6 hours hypoxia 2.86±0.91 ng/L,L\|NNA+6 hours hypoxia 14.75±1,87 ng/L respectively.It was found that hypoxia could induce the expression of VEGF in human umbilical vein endothelial cells; ET could increase the inducible effect of hypoxia,NO could inhibit it and LNNA stimulated the expression of VEGF in human umbilical vein endothelial cells.Read More
Publication Year: 2001
Publication Date: 2001-01-01
Language: en
Type: article
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