Title: A Study on Effects of Several Environmental Factors on Sporulation of Pythium carolinianum, a Fungal Pathogen of Mosquito Larvae
Abstract: Objective: To investigate the suitable environmental conditions needed by Pythium caro-linianum for sporulation. Methods: The amount of zoospores produced by P. carolinianum under different conditions was chosen as the index to reflect the suitability of the fungus to the conditions. Results: The suitable temperature range for sporulation of the fungus were 10 ℃-30℃, while the optimal temperature range was 24 ℃ - 26 ℃ ; The Suitable pH range was 5 - 12, with an optimum range of 9 - 11; Mycelia of 8 - day old had the highest zoospore yield; The optimal ratio of hyphal pieces to water volume(ml) was 1:40(10:400) for zoospore production; The light cycles did not show influence, but ultraviolet had obvious inhibitory effect on sporulation; Zoospore production was significantly reduced by NaCl, (NH4)2SO4, pepton and glucose at the concentrations of 0.2g/l, 50mg/l, 10mg/l, and 10mg/l respectively, but zoospore could be produced when high concentration of urea solution existed. Conclusions: This research has revealed the requirement of P. carolinianum to environmental conditions for sporulation. Data collected here are useful reference for further basic study and application of P. carolinianum.
Publication Year: 2003
Publication Date: 2003-01-01
Language: en
Type: article
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