Title: The Role of Expression of the Cell Apoptosis-relating Genes bcl-2 and bax in Lung Cancer
Abstract: Objective:To study the role of the expression of the apoptosis relating protein bcl 2 and the expression of bax protein with the clinicopathologic charateristic and the survival probablity in human lung cancer cases.Methods:72 resected lung cencer specimens and ll normal lung tissue specimens were investigated by means of immunohistochemisty,the apoptosis status was studied by TUNEL method.Results:The positive expression rates of the bcl 2 and the bax in lung cancer were 37.50% and 51.39%,respectively,they had significant differences in comparison with those in normal lung tissue (P0.05,and P0.01,respectively).The positive expression rate of bcl 2 in small cell lung cancer was 100%(4/4),significantly higher than that in non small cell lung cancer(33.82%,P0.05).The positive expression rate of bcl 2 was 50% in sqamous cell lung cancer,significantly higher than that of in adenocarcinoma of lung(13.33%)(P0.05).The positive expression rate of bax in squamous cell lung cancer was 39.47%,significantly lower than that of 73.33%in adenocarcinomn of lung(P0.05).Analysis of the positive expression rates status of these two gene proteins in non small cell lung cancer cases,our results showed that those ratio of bcl 2/bax1 in well differentiated cancer was 50% among these cases,significantly higher than those of 17.86%in intermediate,lower_,and undifferentiated lung caucer casese(P0.05);it was 39.13% in stage Ⅰ,higher than that of 15.56% in stage Ⅱ Ⅲ cases;and it was 20.00% among those patients survived more than three years,lower than that of 51.16% in those petients suruived less than 1 3 yeers(P0.05).The apoptosis index (AI)was 1.83±0.52 in lung cancer specimens,lower than that of 14.33±5.62 in normal lung tissue (P0.05);among those lung cancer specimens with the bcl 2/bax ratio1,their AI was 1.27±0.49,lower than that of 3.32±1.01 among those with bcl 2/bax ratio1(P0.05).Conclusions:Our results demonstrated that the bcl 2 and bax gene expression was closely related to the histologic types,the degree of differentiation,clinical stage,and the prognosis of lung cancer.These 2 gene proteins play a role in the carcinogenesis and the development of lung cancer.
Publication Year: 2001
Publication Date: 2001-01-01
Language: en
Type: article
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