Title: Expression of MCP-1 in human mesangial cells induced by Ox-LDL is mediated by the activition of nuclear factor-KB
Abstract: Objective To investigate the role of nuclear factor-KB( NF-KB) in the expression of monocyte chemoattractant protein-i (MCP-1) in human mesangial cells induced by oxidized low density lipoprotein(Ox-LDL) . Methods Human mesangial cells were used as target cells. Levels of inhibitory KBK (IKBα) and MCP-1 protein were measured by ELISA. Activity of transcriptional factors NF-KB was determined by electrophoresis mobility shift assay ( EMSA) . Immunohistochemistry was used to detect the translocation of p65. Results Ox-LDL increased the DNA-binding activition of NF-KB and decreased the expression of IKBα. These effects were most obvious when the mesangial cells were cultured with Ox-LDL in 50 μg/ml for 1 hour( P 0.01). The expression of MCP-1 protein in mesangial cells increased at 24 hours when incubated with 10 μg/m1 to 100 μg/ml Ox-LDL and 50 μg/mI Ox-LDL caused the highest expression level. The translocation of p65 from cytoplasm to nucleus was found with the activation of NF-KB too. NF-KB inhibitor pyrrolidithiocabonate( PDTC) could inhibit these effects of Ox-LDL. Conclusion Activition of NF-KB regulates the expression of MCP-1 in human mesangial cells induced by Ox-LDL
Publication Year: 2002
Publication Date: 2002-01-01
Language: en
Type: article
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