Title: Hyperoxic regulation of lung heme oxygenase-1 in neonatal rats
Abstract:Objective Hyperoxia-induced lung injury of neonatal rat model was used to investigate the effect of hyperoxia on lung lesions and to explore the regulation of heme oxygenase-1(HO-1) protein expression...Objective Hyperoxia-induced lung injury of neonatal rat model was used to investigate the effect of hyperoxia on lung lesions and to explore the regulation of heme oxygenase-1(HO-1) protein expression by hyperoxia. Methods Neonatal rats were randomly divided into 7 groups: 0,24,48,72 hours room-air control groups and 24,48,72 hours hyperoxia groups. They were continuously exposed to oxygen(92 %~94 %) and room-air respectively. Their lungs were examined by immunohistochemical staining and the HO-1 protein levels were measured . The lung′s wet/dry weight ratio, histological examination of oxygen induced lung injury were performed.Results Compared with the room-air controls, obvious structural changes, especially the capillary epithelial cells, were seen after 72 hours hyperoxic exposure. The histological changes consisting of edema, hemorrhage and inflammatory were found. There were significantly increases in lung′s wet/dry weight ratio(7.97±1.18 vs 6.40±0.37,P0.05). The mean HO-1 protein expression showed an increase in 72 hours hyperoxia group (0.504±0.062 vs 0.345±0.024,P0.01). HO-1 protein staining was prominently in the bronchiolar epithelium, alveolar wall and macrophage cells.Conclusions Neonatal rats exposed to 92 %~94 % hyperoxia suffered from severe lung injury. Hyperoxic exposure of neonatal rats may induce the up-regulation of HO-1 protein expression. HO-1 may play an important role in the pathophysiology of the pulmonary oxygen injury in neonatal ratsRead More
Publication Year: 2003
Publication Date: 2003-01-01
Language: en
Type: article
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