Title: Construction and analysis of a metagenomic library on phyllosphere microbe
Abstract:The metagenomic library technique is an effective method on the development and utilization of uncultured microorganism gene resources.By a combination of CTAB-SDS method,the genomic DNA of mixed were...The metagenomic library technique is an effective method on the development and utilization of uncultured microorganism gene resources.By a combination of CTAB-SDS method,the genomic DNA of mixed were directly extracted from sediment samples of plant leaf surface.The metagenomic library using puc19 as vector was constructed and 8126 positive clones were obtained.The total number of metagenomic library DNA was about 30.1MB and the average insert size of DNA was about 3.8kb.The successful construction of metagenomic library of phyllosphere microbe would have great significance on studying the relationship between the plant and phyllosphere microbe in future.Read More
Publication Year: 2008
Publication Date: 2008-01-01
Language: en
Type: article
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