Title: Cloning and Prokaryotic Expression of Insect-resistant Gene Cry1Ab/Ac
Abstract: The full-length sequence of integrated Bt gene in genetically modified rice TT51-1,was amplified and cloned into pGEM-T vector. After sequencing was confirmed,the Bt gene was inserted into pET-28a expression vector using HindⅢand BamHⅠ. The recombined pET-28a-cry was transformed into E. coli BL21(DE3)and the recombined target protein(rCRY)was expressed in E. coli BL21(DE3)host cells under 0.1mmol/L IPTG induction. The purified rCRY protein with the relative molecular mass 7.1×104 was recovered from SDS-PAGE gel by denaturation and refolding of inclusive body protein. This work would be helpful for the development of new GMOs detection methods.
Publication Year: 2014
Publication Date: 2014-01-01
Language: en
Type: article
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Cited By Count: 2
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