Title: Content determination of chlorogenic acid in Yanyan Capsules by HPLC
Abstract: Objective:To establish a method for the content determination of chlorogenic acid in Yanyan Capsules by HPLC. Methods:The Kro-masil-C18 (4.6 mm×250 mm,5 μm) column was used. The mobile phases consisted of acetonitrile and 0.4%-phosphoric (70∶30),the flow rate was 1.0 ml/min and the detection wavelength was at 327 nm. Results:The linear ranges of Chlorogenic acid was at 0.424-1.272 μg (r=0.999 7),the average recovery was 99.20% with a RSD of 0.44% (n=5). Conclusion:The method is fast,reliable,accurate and can be used in the quality control of chlorogenic acid in Yanyan Capsules.
Publication Year: 2010
Publication Date: 2010-01-01
Language: en
Type: article
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