Title: Assessment of hemoglobin electrophoresis by CAPILLARYS 2system combined with MCH test in measurement of thalassemia
Abstract: Objective:To evaluate the application value of hemoglobin A2 electrophoresis by CAPILLARYS 2system and mean corpuscular hemoglobin(MCH)in the diagnosis of thalassemia.Method:A total of 275β-thalassemia gene carriers,44αβ-compound thalassemia gene carriers,186α-thalassemia gene carriers and 398non-thalassemia gene carriers were collected from our hospital.MCV and MCH were tested by XE-2100 hemoglobin A2electrophoresis and was analyzed by CAPILLARYS 2system.GAP-PCR and PCR-reverse blot hybridization were used to detect 3commonα-thalassemia genes and 17commonβ-thalassemia genes mutation,respectively.The sensitivity(Se),specificity(Sp),positive predictive value(PV+)and negative predictive value(PV-)were compared between single hemoglobin electrophoresis measurement and combination measurement of hemoglobin electrophoresis with MCH in thalassemia diagnosis.Result:Screeningβ-thalassemia gene carriers andαβ-compound thalassemia gene carriers with hemoglobin electrophoresis by CAPILLARYS 2system,the Se was 100.00%,the Sp was98.29%,the PV+was 96.96%and the PV-was 100.00%.Screeningα-thalassemia gene carriers(including Hb H)with hemoglobin electrophoresis by CAPILLARYS 2system,the Se was 90.32%,the Sp was 72.25%,the PV+was 45.78%and the PV-was 96.64%.Screeningα-thalassemia gene carriers by combination of hemoglobin electrophoresis with MCH,the Se was 90.32%,the Sp was 87.87%,the PV+was 65.88% and the PV-was97.22%.Conclusion:Hemoglobin electrophoresis by CAPILLARYS 2system has high Se and Sp inβ-thalassemia andαβ-compound thalassemia gene carriers screening,while combination of hemoglobin electrophoresis with MCH can improve the Sp and PV+inα-thalassemia gene carriers screening.
Publication Year: 2014
Publication Date: 2014-01-01
Language: en
Type: article
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