Title: The protective effect of thyroid hormone on hepatic ischemia-reperfusion injury in rat
Abstract: Objective:To investigate the protective effect of thyroid hormone on hepatic ischemia-reperfusion injury and its possible mechanism in rat.Methods:The model of 70% hepatic ischemia-reperfusion was established in rat.Sixty-four healthy male SD rats were randomly divided into 4 groups.S:sham operation group;IR:ischemia-reperfusion group;IPC:ischemic preconditioning group;T3:thyroid hormone treatment group.After 6 h and 24 h of reperfusion,aspartate aminotransferase(AST),alanine aminotransferase(ALT) and tumor necrosis factor alpha(TNF-α) levels in the serum were measured;the superoxide dismutase(SOD) activity,levels of malondialdehyde(MDA) and glutathione(GSH) in the hepatic tissue were detected.Histologic changes was observed in HE staining, and the expression of NF-κB in liver cells was evaluated by immunohistochemistry.Results:After ischemia-reperfusion,compared with S group,IR group showed higher levels of ALT,AST,MDA,TNF-αand NF-κB and more obviously injury of liver tissue,but the activity of SOD and GSH decreased.After treatment of thyroid hormone and ischemic preconditioning,all above abnormal parameters were retrieved remarkably(P 0.01).but there are no obvious difference between IPC group and T3 group(P 0.05).Conclusion:Thyroid hormone,as well as ischemic preconditioning,has a protection against hepatic ischemia-reperfusion injury in rat.The mechanism of protection may be associated with its scavenging of radical,inhibition of lipid peroxidation,decrease of TNF-α level and suppression of NF-κB activation.
Publication Year: 2010
Publication Date: 2010-01-01
Language: en
Type: article
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