Title: Genetic variation analysis of 5a,5b and N genes of infectious bronchitis virus HN strain
Abstract: The infectious bronchitis virus (IBV) HN strain was successfully isolated from a chicken flock with typical clinical symptom of infectious bronchitis in Henan Province of China. The strain was passaged in SPF chicken embryos, characterized by haemagglutination test and observed by electron microscope. The cDNA fragment including nucleocapsid protein gene, 5a and 5b protein genes of the strain, which is about 1600bp in length, were amplified according to designed specific primers by RT-PCR from viral RNAs. Compared the 5a, 5b genes and N gene respectively with that of the other 11 IBV strains from GenBank on genetic variation, homology of nucleotide and deduced amino acid sequences the IBV HN strain differed from the other strains from GenBank obviously.
Publication Year: 2005
Publication Date: 2005-01-01
Language: en
Type: article
Access and Citation
AI Researcher Chatbot
Get quick answers to your questions about the article from our AI researcher chatbot