Title: Determination of Notoginsenoside R1, Ginsenoside Rg1 and Ginsenoside Rb1 in Rupixiao Granule by HPLC
Abstract: OBJECTIVE To establish an HPLC method for determination of notoginsenoside R1,ginsenoside Rg1 and ginsenoside Rb1 in Rupixiao granule.METHODS The chromatographic conditions included C18 column(CAPCELL PAK,150 mm×4.6 mm,5 μm),acetonitrile as mobile phase A,water as mobile phase B.The flow rate was 1.0 mL.min-1.The detection wavelength was 203 nm.Gradient elution: 0.12 min,A: 19%,B: 81%;12-60 min,A:19%→36%,B: 81→64%.RESULES The retention time of notoginsenoside R1,Ginsenoside Rg1 and Ginsenoside Rb1 was 21,25,51 min,respectively.The resolution was above 1.5.The linear range of notoginsenoside R1,ginsenoside Rg1 and ginsenoside Rb1 was 0.100 2-2.004 μg,0.418 8?8.376 μg and 0.187-3.74 μg.The average recovery was 98.5%,99.6% and 96.5%,RSD was 1.9%,1.3% and 1.9%,respectively.CONCLUSION The method is simple,accurate and reproduciable.It can be used for routine analysis of notoginsenoside R1,ginsenoside Rg1 and ginsenoside Rb1 in Rupixiao granule simultaneously.
Publication Year: 2010
Publication Date: 2010-01-01
Language: en
Type: article
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