Title: The Comparative Research on Brain Protection of Different Concentrations of Hypertonic Saline on Acute Focal Cerebral Ischmia-reperfusion Injury
Abstract: Objective To explore the protetcive effect of four different concentrations of hypertonic saline on cerebral ischemia and reperfusion and done a comparative analysis to explore the optimal concentration of hypertonic saline and provide experimental evidence for the treatment of cerebral Ischemia-reperfusion injury.Methods Thread embolism was performed to made the acute focal cerebral ischemia and reperfusion model,at 10 minutes before ischemia and 12 hours after reperfusion,intravenous 0.9% NaCI 4 mL/kg(A group),7.5% NaCI 4 mL/kg(B group),10% NaCI 4 mL/kg(C group);23.5% NaCI 4 mL/kg(D group),all rats were graded scores of neurobehavioral function at 2 hours after surgery anesthesia,6 hours and 24 hours after reperfusion.Then the infarct size was measured after stained by TTC;the water content of both hemispheres was calculated;the number of neutrophil at a continuous five fields was counted by HE staining.Results The neurobehavioral scores and infarct volume had no significant difference between groups(P0.05).D group's brain water content was less than the other group(P0.05),the infiltration of neutrophils at infraction was also no significant difference between groups(P0.05).Conclusions The dehydration of 23.5% Nacl is better than the other three concentrations Nacl.There is no significant difference in the infarct volume of Ischemia-reperfusion brain,infarct inflammation and the score of neurobehavioral function among the four concentrations of Nacl.
Publication Year: 2012
Publication Date: 2012-01-01
Language: en
Type: article
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