Title: Protective Effect of Danhong Injection on Ischemia-reperfusion Injury in Rats After Kidney Transplantation
Abstract: Objective To investigate the protective effect of Danhong Injection(DI) on rats ischemia-reperfusion(I/R) injury after kidney transplantation.Methods Ten normal SD rats were allocated into the sham-operation group,and they were only been anesthetized and exposed kidneys for 45 minutes.The allogeneic male SD rats model of kidney transplantation were established.The qualified rats were divided into 3 groups(10 in each group)at random:I/R model group,DI pretreatment group,and DI treatment group.B1ood urea nitrogen(Bun) and creatinine(Cr) levels in each group were determined at the 24th hour after operation.The expression of Bax and Bcl-2 in kidney were quantitatively detected by immunohistochemistry.Apoptotic index was measured by TUNEL method.Results Compared with the sham-operation group,the apoptosis rate(P 0.01) and the expression of Bax and Bcl-2(P 0.01) were significantly increased in the other 3 groups,and the Bcl-2/Bax ratio(P 0.01) was decreased significantly in I/R model group.Compared with those in I/R model group and DI treatment group,the apoptosis rate(P 0.01) and the expression of Bax were decreased significantly(P 0.01),the expression of Bcl-2(P 0.01) and the Bcl-2/Bax ratio(P 0.01) were increased significantly in DI pretreatment group.Conclusion Danhong injection has protective effect on Renal ischemia-reperfusion injury after kidney transplantation,and its mechanism may be related to the expression regulation of cell apoptosis gene Bcl-2/Bax.
Publication Year: 2009
Publication Date: 2009-01-01
Language: en
Type: article
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