Title: Evaluation of a rat model of bladder dysfunction induced by complete spinal cord transection
Abstract: Objective:To establish and evaluate bladder dysfunction following complete suprasacral spinal cord transection in a standardized rat model.Method:Sixteen adult female Sprague-Dawley rats were operated at T9—T11 vertebra,after laminectomy rat's suprasacral spinal cords were completely transected.After surgery daily weight of expressed urine was measured and recorded to assess the changes of bladder activity.Measurements of continuous uroflow cystometry were performed and compared between intact rats and spinal cord transected rats.Six healthy rats were used as controls.Result:Most of the complications could be relieved with post-surgery care.The weight of urine increased initially and reached to a peak at 1-week post-spinal-transection followed by gradually declining.Under continuous infusion of saline into the bladder with constant rate,the bladder of intact rats initiated periodic voiding contractions.In contrast,the bladder of spinal-transection rats showed uninhibited non-voiding contractions with increasing pressure and smaller void contractions at larger bladder volumes with high and sustained intravesical pressures.Conclusion:In the recovery period the spinal cord transected rats had detrusor-external sphincter dyssynergia.The rat model with bladder dysfunction following complete suprasacral spinal cord transection was successfully established.The rat model could be used to investigate the efficacy of sacral-neuromodulation for treating bladder dysfunction in future studies.
Publication Year: 2012
Publication Date: 2012-01-01
Language: en
Type: article
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