Title: Antagonizing effect of glucocorticoid on SEB-induced immune tolerance in high-risk corneal transplantation in rats
Abstract: AIM: To study the antagonizing effects of glucocorticoid on the immune tolerance induced by staphylococcal enterotoxin B(SEB) and the mechanism involved. METHODS: High risk corneal transplantation was performed using rats with different minor histocompatibility antigens. Lewis rats were used as the receptor and F344 as the donor. Suture was used to make high risk corneal transplantation model. Before and after corneal transplantation, peribulbar injection of SEB or SEB plus glucocorticoid was performed. After surgery, rejection index (RI) and the survival time of the corneal grafts were recorded using a slit lamp microscope. Response of lymphocytes and the percentage of sub population of lymphocytes in the recipient spleens, peripheral blood and submandibular gland were determined using flow cytometry and mixed lymphocyte culture technique. RESULTS: Peribulbar SEB of proper dose increased the survival time of corneal grafts and decreased the immune response of mixed cultured lymphocytes. The fraction of CD4+/NK1.1 +NKT cells was significantly increased in the spleen, peripheral blood and submandibular gland of the receptor from 0 05 to 0 17, 0 02 to 0 12 and 0 02 to 0 07 respectively. But the survival time of corneal grafts in the group receiving SEB plus glucocorticoid was not increased. The percentage of CD4+/NK1 1 +NKT cells in various tissues was decreased. CONCLUSION: SEB plus glucocorticoid cannot increase the survival time of corneal graft in high risk corneal transplantation. Glucocorticoid antagonizes the immune tolerance induced by SEB. This decreased immune tolerance may be correlated with the down regulation of CD4+/NK1.1+NKT cells by glucocorticoid.
Publication Year: 2003
Publication Date: 2003-01-01
Language: en
Type: article
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