Title: Polymorphic short tandem repeats for diagnosis of the Charcot-Marie-Tooth 1A duplication
Abstract:Objective:Short tandem repeats (STRs) sequences 4A, 9A and 9B were used to detect gene dup lication of Charcot Marie Tooth disease type 1A(CMT1A). Methods: Polymorphism and heterozygosity of STR 4A, 9...Objective:Short tandem repeats (STRs) sequences 4A, 9A and 9B were used to detect gene dup lication of Charcot Marie Tooth disease type 1A(CMT1A). Methods: Polymorphism and heterozygosity of STR 4A, 9A and 9B were analyzed in 100 nor mal controls using polymerase chain reaction and Single strand conformational p olymophisms (PCR SSCP). Then STR 4A, 9A and 9B were used to detect gene duplica tion in 30 unrelated CMT1 patients. Results: The polymorphi sm of the 3 markers is 8, 12 and 11 respectively. The heterozygosity of them is 0 75, 0 84 and 0 81 respectively. 60%(18/30) of CMT1 patients were detected gene duplication using 3 markers. The rate is higher than that(52 9%) which was detected by the primary markers in our laboratory. Conclusion:B oth polymorphism and heterozygosity of STR 4A, 9A and 9B are high in normal cont rols and in CMT1 patients. Especially 9A and 9B have the highest observed hetero zygosity reported to date for CMT1A markers. They are the ideal markers to detec t CMT1A gene duplication clinically.Read More
Publication Year: 2003
Publication Date: 2003-01-01
Language: en
Type: article
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