Title: Content comparison of ursolic acid and oleanolic acid among Xiakucao(Spica Prunellae) produced from different areas
Abstract: Objective To determine the content of ursolic acid and oleanolic acid in Xiakucao(Spica Prunellae) produced from different areas.Methods The HPLC procedure was performed on the chromatographic column of Diamonsil C18(4.6 mm×250 mm,5 μm),and the mobile phase was methanol-0.5% phosphoric acid solution(86∶ 14) in gradient elution.The flow velocity was 1.0 mL/min,the detection wavelength was 210 nm and the temperature was 35 ℃.Results Ursolic acid showed a good linear relationship with peak area at range of 0.416-13.312 μg(r=0.999 5) and the average recovery was 98.19%(RSD=2.86%,n=6).Oleanolic acid showed a good linear relationship with peak area at range of 0.464-4.640 μg(r=0.999 8),and the average recovery was 97.56%(RSD=2.08%,n=6).The content of ursolic acid and oleanolic acid was,respectively,between 0.155 3% to 0.256 3% and 0.039 6% to 0.039 6% in Xiakucao produced from different areas.Conclusion The content of ursolic acid and oleanolic acid is different in Xiakucao produced from different areas,which should be paid attention to during administration.
Publication Year: 2011
Publication Date: 2011-01-01
Language: en
Type: article
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