Title: Study on Tissue Culture and Plantlet Regeneration of Codonopsis lanceolata
Abstract: Different explants were used to study the influences of different hormone combinations,media and illumination conditions on callus induction and plant regeneration of Codonopsis lanceolata.The results showed that leaf was the optimal explant and the optimal medium for callus induction was MS medium adding 0.5 mg·L-1 2,4-D,1.0 mg·L-1 6-BA and 0.5 mg·L-1 KT on which the callus-forming rate could reach to 100%.After subcultured on MS medium with 0.75 mg·L-1 6-BA and 0.5 mg·L-1 NAA,the callus were differentiated on 1/2MS medium with 0.5 mg·L-1 6-BA and 0.2 mg·L-1 NAA,and the differentiation rate could reach to 89.4%.1/2MS medium with 0.2 mg·L-1 IAA and 0.2 mg·L-1 NAA was the best for rooting on which the rooting rates could reach to 92.5%.After inducted in darkness,the multiplication times and differentiation rate of callus were greatly increased when incubated with illumination of 16 h·d-1.Regenerated plants were strong and grew quickly.
Publication Year: 2010
Publication Date: 2010-01-01
Language: en
Type: article
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Cited By Count: 1
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