Title: Effect of aspirin on apoptosis of ovarian carcinoma cells and expression of Bcl-2/bax mRNA
Abstract: Objective To investigate the effect of aspirin on apoptosis of ovarian carcinoma cells and expression of Bcl-2/bax mRNA.Methods Morphologic changes of apoptotic cells were observed by optical microscope after Giemsa staining.Cell apoptosis and cell cycle was detected by cell flow cytometry(FCM).The expression of Bcl-2/bax mRNA was detected by RT-PCR.Results Morphology changes of apoptosis and apoptotic body were found by optical microscope.The apoptosis percentage(AP) induced by aspirin was detected by FCM,which showed that aspirin at dosage of 1,5,10 mmol/L for 48 h could induce cell apoptosis in a dose-dependent way ranging from(11.1±0.5)%,(17.9±0.4)%,(30.8±0.4)% respectively(P0.01).There were visible apoptotic peaks of hypodiploid on the DNA bargraph.Compared with control group,after treated with aspirin,SK-OV-3 cells of G0/G1 stage decreased and cells of G2/M stage increased.Aspirin could down-regulate the expression of Bcl-2 mRNA,up-regulate the expression of bax mRNA.Conclusion Aspirin can inhibit the proliferation of ovarian carcinoma cells and induce its apoptosis,and which can induce the apoptosis of SK-OV-3 cells by down-regulating the expression of Bcl-2 mRNA,and up-regulating the expression of bax mRNA,so that aspirin has the effect of antineoplasna.
Publication Year: 2009
Publication Date: 2009-01-01
Language: en
Type: article
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