Title: Expression and identification of Helicoverpa armigera cathepsin B in baculovirus system
Abstract: Recombinant cloning vectors with Helicoverpa armigera cathepsin B gene were constructed.After being isolated,the recombinant bacmids were used for transfection into Sf21 cells with lipofectin.The supernatant of transfection was used for infection into Sf21 cells.From the supernatant,budded virus was extracted in order to identify HCB gene by PCR.The supernatant was analyzed by assays of SDS-PAGE,western-blotting and protease activity analysis.The result of PCR shows that DNA fragment of 1?700?bp has been obtained which is correlated to the length of HCB gene and the vector arm.A new protein with MW 28 ku has been found in the supernatant by SDS-PAGE and western-blotting and it also shows proteolysis activity in the experiment of protease activity.
Publication Year: 2005
Publication Date: 2005-01-01
Language: en
Type: article
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