Title: Establishment of a rat model of general ischemia-reperfusion injury
Abstract: Objective:To establish a rat model of general ischemia-reperfusion injury.Methords:Thirty Wistar rats achieved ischemia by hemorrhage and achieved reperfusion iniury by infusion of the bled blood.1 hour after hemorrhage the total bled blood was reinfused into the animals and the rats were killed 3 houes later.Mean arterial pressure(MAP),heart rates,anus temperature and electrocardiogram(ECG) were monitored all the time.Blood samples were taken before hemorrhage and after 3 hours of inspection were analyzed for serum aspartate aminotransferase(AST),alanin aminotransferase(ALT),lactate dehydrogenase(LDH).Heart,kidney,brain and lung samples were taken for histopathological analysis.Results:The results showed that data of MAP,heart rates,anus temperature were normal distribution.Compared with those before hemorrhage the AST,ALT,LDH increased significantly after 3 hours of reperfusion iniury.The changes of ECG after ischemia and reperfusion accorded with ischemia and reperfusion injury.Histopathological changes of heart,kidney,brain and lung after 3 hours of reperfusion accorded with reperfusion injury.Conclusion:A rat model of general ischemia-reperfusion injury was successfully established.
Publication Year: 2011
Publication Date: 2011-01-01
Language: en
Type: article
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