Title: Study of salidroside protects cultured SH-SY5Y cells against β-amyloid peptides injury
Abstract:Objective: To investigate the effect of salidroside on β-amyloid(Aβ) SH-SY5 Y cells induced injury and its possible mechanism. Methods: SH-SY5 Y cells were cultured in vitro, using cell culture medium...Objective: To investigate the effect of salidroside on β-amyloid(Aβ) SH-SY5 Y cells induced injury and its possible mechanism. Methods: SH-SY5 Y cells were cultured in vitro, using cell culture medium containing Aβ1-40, for preparing disease models, cultured with Aβ1-40 for 24 h, different concentrations of salidroside(100 and 200μmol/L) were used for the cure, MTT(Tetramethyl triazole salt) assay was used for checking the cell viability; Flow cytometry, TUNEL were used for the detection of intracellular reactive oxygen species ROS and apoptosis; The detection of intracellular AGEs content by ELISA; The detection of intracellular advanced glycation end products receptors RAGE, NF-κB(p-p65), Bax and Bcl-2 protein expression changes were used by Western blot. Results: Salidroside intervention can signii cantly improve the cell viability after injuried by Aβ1-40, inhibit the increased intracellular ROS level and apoptosis rate induced by Aβ1-40; it can reduce expression of RAGE, NF-κB(p-p65), also reverse Bcl-2/Bax protein ratio decline induced by Aβ1-40. Conclusion: Salidroside can inhibit the damage and apoptosis of SH-SY5 Y cells induced by Aβ1-40, which may be related adjustment of ROS, AGEs/RAGE/NF-κB axis and apoptosis related genes Bax and Bcl-2 protein expression.Read More
Publication Year: 2014
Publication Date: 2014-01-01
Language: en
Type: article
Access and Citation
AI Researcher Chatbot
Get quick answers to your questions about the article from our AI researcher chatbot