Title: Role of miR-486-5p in apoptosis of human bone marrow mesenchymal stem cells induced by hydrogen peroxide
Abstract:AIM: To investigate the role of microRNA-486-5p(miR-486-5p) in the apoptosis of human bone marrow mesenchymal stem cells( h MSCs) induced by hydrogen peroxide( H2O2). METHODS: The h MSCs were cultured...AIM: To investigate the role of microRNA-486-5p(miR-486-5p) in the apoptosis of human bone marrow mesenchymal stem cells( h MSCs) induced by hydrogen peroxide( H2O2). METHODS: The h MSCs were cultured in vitro and exposed to serum-free medium and H2O2(10 mmol/L). The changes of miR-486-5p expression in oxidative stress-related apoptosis of h MSCs were measured by real-time PCR. The h MSCs were transfected with miR-486-5p mimic or inhibitor at concentration of 30 nmol / L by Lipofectamine RNAi MAX. The effect of miR-486-5p on H2O2-induced decrease in cell viability was evaluated by MTT assay. Hoechst 33342 staining and flow cytometry were applied to determine the role of miR-486-5p in the apoptosis of h MSCs. The protein expression was evaluated by Western blotting. Caspase-3 activity was determined using a caspase-3 activity kit. RESULTS: Compared with control group,the expression of miR-486-5p significantly decreased after treated with H2O2(P 0. 05). In addition,over-expression of miR-486-5p in the h MSCs reduced the cell viability,accelerated apoptosis,down-regulated Bcl-2 / Bax ratio,caspase-3 enzyme precursor content and phosphorylation of Akt,and activated caspase-3 activity. Conversely,down-regulation of miR-486-5p significantly inhibited H2O2-induced cell apoptosis and the caspase-3 activity,increased cell viability and up-regulated Bcl-2 / Bax ratio and phosphorylation level of Akt. CONCLUSION: Over-expression of miR-486-5p promotes H2O2-induced h MSCs apoptosis,and repression of miR-486-5p protects h MSCs from H2O2-induced cellular apoptosis,which may be mediated by regulating Akt signaling pathway.Read More
Publication Year: 2015
Publication Date: 2015-01-01
Language: en
Type: article
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Cited By Count: 1
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