Title: Optimization Extract of Chlorogenic Acid from Lonicera Japonica Thunb. by Means of Cellulase
Abstract: The effects of cellulase contents,cellulase hydrolyzation temperature,cellulase hydrolyzation time,refluxing temperature on the extraction of chlorogenic acid in Lonicera japonica Thunb.were investigated by means of optimizing extraction with enzyme treatment.High performance liquid chromatography(HPLC) was used to determine the content of chlorogenic acid.The method of extraction with enzyme in Lonicera japonica Thunb.could enhance the yield of chlorogenic acid.The optimal condition is 3.0% cellulase,cellulase hydrolyzation temperature 46℃,cellulase hydrolyzation time 4 h,and 1 h for leaching at temperature of 56℃.The content of chlorogenic acid extracted from Lonicera japonica Thunb.was 3.57%.
Publication Year: 2007
Publication Date: 2007-01-01
Language: en
Type: article
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