Title: The Effects of the Prepared Aconite Lateral Root and the Prepared Aconite Lateral Root Combined with Dried Ginger on Renal Function of Mice with Chronic Renal Failure Induced by Adenine
Abstract: Objective:To observe the therapeutic effects of the prepared aconite lateral root and the prepared aconite lateral root combined with dried ginger on chronic renal failure(CRF) in mice,and to explore the related mechanisms preliminarily.Methods:Male ICR mice were randomly divided into 6 groups including control group,model group,low dose group of prepared aconite lateral root(FL group) and high dose group(FH group),as well as low dose group of prepared aconite lateral root combined with dried ginger(FLG group) and high dose group(FHG group).The mice in control group and the other groups were given distilled water and adenine(250 mg ·kg-1,every two days in the first week;130 mg ·kg-1,every two days from the second week to the fourth week) by intragastric administration,respectively.The mice in FL group,FH group,FLG group and FHG group were given the corresponding water extracts by intragastric administration once daily.The general conditions of the mice were observed and the contents of creatine(Cr),urea nitrogen(BUN),lactic acid(LD) and lactate dehydrogenase(LDH) in blood serum were determined.The kidneys were homogenated for measuring glutathione(GSH),alanine transaminase(ALT),aspartate amino-transferase(AST) and malonaldehyde(MDA).Besides,the number of sperm in the left epididymis of mice was recorded.Results:Compared with control group,the body weight and food intake of mice in model group and test groups decreased significantly,with the drinking amount increased.The drinking amount of mice in FH group and FLG group was lower than that of model group.The mice in model group became emaciated,weary and clothing hair-kraurotic,and some died during the experimental period.The mice in FHG group and FLG group had tramosericeous clothing hair,and they were more active.Compared with model group,the biochemical indicators of mice in FH group,FLG group and FHG group were improved significantly,with the blood Cr,BUN,LD and LDH reduced,and sperm count was raised.Compared with model group,the GSH and MDA levels in the nephridial tissue of mice were reduced and raised respectively in the above groups.Moreover,the ALT level of nephridial tissue in FH group and FHG group,as well as the AST level in FLG group and FHG group were higher than those of model group.The biochemical indicators in FL group were not improved,except for a few indexes.Conclusion:The water extract from prepared aconite lateral root of high dose can protect the renal function of mice with chronic renal failure induced by adenine.Dried ginger can enhance the effects of the prepared aconite lateral root,and reduce its toxicity.
Publication Year: 2011
Publication Date: 2011-01-01
Language: en
Type: article
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Cited By Count: 2
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