Title: Effect of Bombesin on growth of human gastric cancer cell line BGC-823
Abstract:Objective:To observe the effect of Bombesinon on the growth of a poorly differentiated human gastric cancer cell line BGC-823 and to explore the post-receptor signal transduction pathway by determinin...Objective:To observe the effect of Bombesinon on the growth of a poorly differentiated human gastric cancer cell line BGC-823 and to explore the post-receptor signal transduction pathway by determining the content of intracellular cyclic adenosine monophosphate(cAMP),the activity of protein kinase C(PKC),and the expression of PKC isoforms α,β1,β2,and e.Methods:BGC-823 cells were cultured in RPM1 1640 medium containing 10% FCS in atmosphere of 5% carbon dioxide at 37℃,and then were treated with different concentrations of Bombesin or its antagonist.The viability and proliferation were determined with MTT assay.The content of intracellular cAMP was detected by radioimmunoassay.The activity of PKC was measured by incorporating [γ-32p]-ATP into exogenous substrate,and the expressions of PKC isoforms was analyzed with Western blotting.Results:Bombesin promoted the growth of BGC-823 cells in a dose-dependent manner,which can be inhibited significantly by the antagonist of Bombesin.Bombesin increased the content of intracellular cAMP and the activity of PKC.The expression of PKC-α significantly increased,but no expression of PKC-β1,β2,and e was found.Conclusion:Bombesin may play an important role in the proliferation,production of intracellular cAMP,and activity of PKC in poorly differentiated gastric carcinoma cell line BGC-823.Read More
Publication Year: 2007
Publication Date: 2007-01-01
Language: en
Type: article
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