Title: Establishment of CHO cell line stably overexpressing human wild type DNA polymerase beta gene and its biological characteristics
Abstract: A recombined enhanced green fluorescent protein(GFP) vector pEGFP-AC3 carrying wide type DNA pol βgene was transfected into CHO cells by lipotransfection,and the stable transfectants were screened by G418.Stable overexpression of pol β in CHO was confirmed by RT-PCR,and its biological characteristics were analyzed from cell cycle,spontaneous mutation and cell soft agar growth.Expression level of pol β in the transfected cell increased significantly than the controls.The cell cycle distribution changed and most cells remained at S phage.And there was significant change in soft agar growths.Its spontaneous mutation rate was higher than that of the control cells.Overexpression of wild type pol β has some effect on the cell cycle distribution,malignant proliferation ability and genetic stability of cells.
Publication Year: 2006
Publication Date: 2006-01-01
Language: en
Type: article
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