Title: Construction and Detection of Retrovirus Vector with Mouse Sox2 gene and GFP Gene
Abstract: In order to establish the method detecting of Sox2 gene expression during somatic cells reprogramming,this study cloned mouse Sox2 gene,and linked with retroviral vector pMX-IRES-GFP.293GP cells were transfected with the vector and pVSV-G vector,mouse fibroblasts(NIH3T3 cells) were infected by viral supernatant.GFP expression was observed and the amount of Sox2 transcription was tested after infection.The results were showed as followed.The retroviral vector pMX-Sox2-IRES-GFP was constructed successfully,viral supernatant was obtained after the vector and pVSV-G co-transfection.Compared to negative control infected NIH3T3 cells expression of Sox2 increased nearly 10-fold.These results would be used for promoting somatic cells reprogramming study.
Publication Year: 2012
Publication Date: 2012-01-01
Language: en
Type: article
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